cd34 + bone marrow mononuclear cells (bmncs) (Lonza)
Structured Review

Cd34 + Bone Marrow Mononuclear Cells (Bmncs), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd34+++bone+marrow+mononuclear+cells+%28bmncs%29/pmc05349961-110-0-16?v=Lonza
Average 90 stars, based on 1 article reviews
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1) Product Images from "VENTX induces expansion of primitive erythroid cells and contributes to the development of acute myeloid leukemia in mice"
Article Title: VENTX induces expansion of primitive erythroid cells and contributes to the development of acute myeloid leukemia in mice
Journal: Oncotarget
doi: 10.18632/oncotarget.13563
Figure Legend Snippet: A . Heatmap of differentially expressed genes as determined by RNA-seq between CD34 + CB cells overexpressing VENTX compared to the empty vector control (n=3). B . Differentially expressed genes transcription factors, which are known to play a role in erythroid development. ****: p<0.0001, ***: p≤0.0001, **: p≤0.001. C . Gene set enrichment analysis of genes involved in erythropoietic differentiation.
Techniques Used: RNA Sequencing, Plasmid Preparation, Control
Figure Legend Snippet: A . Quantitative expression of VENTX in different AML cell lines compared to BM CD34 + /BMNCs/BM GlyA + /PB GlyA + . All expression analyses were performed by TaqMan ® qRT-PCR with (+)RT and (-)RT reaction samples. Fold expression values were obtained by normalizing the expression of the gene of interest (VENTX) to the endogenous human β-actin (β-Act). Bars are showing the average fold expression ± SEM. *: p≤0.05, **: p≤0.001, ***: p≤0.0001, ****: p<0.0001. B . Mean Quantitative expression of VENTX AML M6 and PML-RARα positive AML samples compared to Glycophorin A from BM/PB and CD34 + bone marrow cells. All expression analyses were performed by TaqMan ® qRT-PCR with (+)RT and (-)RT reaction samples. Log 2 fold expression values were obtained by normalization of the expression of the gene of interest (VENTX) to the endogenous human β-actin. Bars are showing the log 2 fold expression ± SEM. **: p≤0.001 and ****: p<0.0001. ○ indicates no detectable expression for 5 individual samples of PML-RARα positive AML cases (for up to 37 cycles).
Techniques Used: Expressing, Quantitative RT-PCR